NTISthis.com

Evidence Guide: MSL975057 - Perform histological tests

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL975057 - Perform histological tests

What evidence can you provide to prove your understanding of each of the following citeria?

Receive and check specimens and associated request forms

  1. Identify specimens and request forms that do not comply with workplace requirements
  2. Record any discrepancies and indicate action required
  3. Register samples in accordance with workplace procedures, recording details that allow accurate tracking and chain of custody traceable to employee
  4. Prepare work area for safe and effective workflows
  5. Perform pre-use checks to ensure processor is fit for purpose
  6. Inspect processor reagents for deterioration and adequate volume, and report any items requiring replacement
  7. Record any issues that may impact on sample integrity and indicate action required
  8. Check reagents are in sequential order
  9. Select processor program for routine and non-routine samples
  10. Perform pre-use check to ensure embedding centre is fit for purpose and uninterrupted embedding of processor load
  11. Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy
  12. Apply procedures to prevent cross-contamination between tissues
  13. Inspect blocks, reject and re-embed items that do not meet quality control standards
  14. Label required number of microscope slides in accordance with workplace traceability requirements
  15. Check the flotation bath is ready and satisfactory for use
  16. Prepare and adjust all external parts of a microtome and associated equipment for routine sample processing
  17. Secure block in microtome following specified safety directions
  18. Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts
  19. Cut representative sections at the required thickness according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing
  20. Float sections onto water bath to flatten tissues and pick up sections onto microscope slides ensuring patient identification on slides matches that on the block
  21. Compare the blocks and sections ensuring the tissue on slides matches that in the blocks
  22. Apply procedures to prevent cross-contamination between patient tissues
  23. Identify, troubleshoot and resolve common section quality control issues
  24. Inspect sections and reject any that do not meet quality control standards
  25. Prepare any labile reagents for immediate use
  26. Select reagents for specified technique, ensuring reagent sequence matches workplace procedure
  27. Stain sections in accordance with workplace procedures using the required quality control section and accommodating any authorised variations
  28. Mount slides using medium compatible with staining technique ensuring no bubbles
  29. Examine control sections microscopically to ensure expected staining outcomes are achieved
  30. Confirm microscopically that the type/disease is appropriate and still present in the control sections for each stain
  31. Label specimens in accordance with workplace traceability requirements
  32. Collate, approve and release slides to pathologist in accordance with workplace procedures
Identify specimens and request forms that do not comply with workplace requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Record any discrepancies and indicate action required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Register samples in accordance with workplace procedures, recording details that allow accurate tracking and chain of custody traceable to employee

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare work area for safe and effective workflows

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform pre-use checks to ensure processor is fit for purpose

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect processor reagents for deterioration and adequate volume, and report any items requiring replacement

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Record any issues that may impact on sample integrity and indicate action required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check reagents are in sequential order

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select processor program for routine and non-routine samples

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform pre-use check to ensure embedding centre is fit for purpose and uninterrupted embedding of processor load

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect blocks, reject and re-embed items that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Label required number of microscope slides in accordance with workplace traceability requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check the flotation bath is ready and satisfactory for use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare and adjust all external parts of a microtome and associated equipment for routine sample processing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Secure block in microtome following specified safety directions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cut representative sections at the required thickness according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Float sections onto water bath to flatten tissues and pick up sections onto microscope slides ensuring patient identification on slides matches that on the block

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Compare the blocks and sections ensuring the tissue on slides matches that in the blocks

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply procedures to prevent cross-contamination between patient tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify, troubleshoot and resolve common section quality control issues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inspect sections and reject any that do not meet quality control standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare any labile reagents for immediate use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select reagents for specified technique, ensuring reagent sequence matches workplace procedure

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Stain sections in accordance with workplace procedures using the required quality control section and accommodating any authorised variations

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Mount slides using medium compatible with staining technique ensuring no bubbles

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Examine control sections microscopically to ensure expected staining outcomes are achieved

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Confirm microscopically that the type/disease is appropriate and still present in the control sections for each stain

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Label specimens in accordance with workplace traceability requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Collate, approve and release slides to pathologist in accordance with workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain laboratory records

  1. Complete control stain quality assessment forms to report quality control outcome
  2. File and store samples to facilitate efficient retrieval as required
  3. Maintain instrument logs as required by accreditation checks
Complete control stain quality assessment forms to report quality control outcome

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

File and store samples to facilitate efficient retrieval as required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain instrument logs as required by accreditation checks

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge